The above sequence shows a partial sequence of the lac operon which contains the

Question

The above sequence shows a partial sequence of the lac operon which contains the following elements. CRP Binding site -35 and -10 promoter operator binding site sequence the transcriptional start site RBS-ribosomal binding site The initial 58 bps of the Lac Z coding sequence (CDS)- in other words the sequence that encodes for lac Z. Use the above information to answer the following: What would be the first 20 bases, starting from the 5' end of the mRNA sequence? What is the purpose of the -35 and -10 and what protein binds to it? What is the meaning behind numbers? What is the RNA sequence of the 5'UTR (untranslated region). What is the RNA sequence of the ribosomal binding site.

Explanation / Answer

B) -35 and -10 sequences are the consensus sequences of the prokaryotic promoter where RNA polymerase (sigma 70) binds that initiates transcription. -10 seq is also known as pribnow box that has consensus TATAAT and -35 has consensus TTGACA.

Related Questions

Navigate

• Browse (All)
• Browse T
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.