During the process of transcription, the RNA polymerase II carboxyl terminal dom

Question

During the process of transcription, the RNA polymerase II carboxyl terminal domain (CTD) is phosphorylated by specific kinases on serine amino acids in a 'heptad repeat'. Specific phosphatases can remove these phosphate groups. Which of the following phosphatases do you think would be most effective in immediately stopping any new transcription when added to an in vitro transcription reaction (a test tube reaction containing RNA polymerase II, a DNA template, rNTPs and all of the general transcription factors)? Explain. phosphatase specific for Ser-2 phosphatase specific for Ser-5

Explanation / Answer

transcription is a method in which RNA poymerase attaches itself to the begining of a gene on DNA and synthesises m-RNA, using one of the strands in DNA as a template. this processes is known as transcription. RNAPII exits two forms : RNAPII0 and RNAPIIA. phosphorylation occurs principally on ser 2 and ser5 of the repeats. the phosphorylation state changes as RNAPII progresses through the transcription cycle like initiating RNAPII is form IIA, and the elongating enzyme is form II0. ser5 phosphorylation 5 startes at initial site causes move RNAP complex move away from initial site, then ser2 comes into action. answer is phosphotase specific for ser5

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