2.Name three pathways or methods of cell death, as well as two \"non-death\" cyt

Question

2.Name three pathways or methods of cell death, as well as two "non-death" cytotoxic effects cells may experience.

3.What are a few limitations of in vitro assessment of cell viability or cytotoxicity?

4.Define and describe the following classes of cell viability assays; give an example of each:

i.cytolysis or membrane leakage assays

ii.mitochondrial activity (caspase assays)

iii.functional assays

iv.genomic or proteomic assays

5.How can Trypan blue be used to estimate cell viability while counting on a hemocytometer?

6.Briefly, explain the theoretical premise of how the Live / Dead assay works.

Please answer the questions fully before i'd rate it. Thanks

Explanation / Answer

2. The three pathways of cell death are apoptosis , autophagy and necrosis .

Apoptosis is type I cell death which is a programmed cell death. Autophagy is type II cell death, which is also programmed . Necrosis is the cell death caused due to external factors such as trauma, injury or infection.

Non death cytotoxic events in reponse to toxic agents that a cell may experience are rapid swelling, loosing membrane integration , stoppage of cell divisions ( decrease in cell viablility) ,etc

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