The buffer or solution is used to move the compounds from a column is called voi

Question

The buffer or solution is used to move the compounds from a column is called void volume elution volume bad volume mobile phase We need to make an anion exchange column, which of the following charge group would be the approximate group to tag beads as station art phase Carboxylic acid (negative charge) Phosphate (negative charge) group (positive charge) a, and b and c Using above column, which protein would be eluted last Positive net charge weak Positive net charge negative net charge neutral (no net charge) Using above column, how many distinguishable fractions you would get at the end of fractionation 1 4 3 2 in a G-75 column (fractionation range 3000-80,0000 D), determine the order of elution of following proteins (first to last) C (MW:13,000 D), Myoglobin (MW:16, 300 D), Serum albumin (MW:68,000 0), Catalase (MW:222,000 0), Unknown protein (MW: 400,000 D0 Catalase MW:222 KD and Unknown protein MW: 400 KD (elute together), Serum albumin MW:68 KD, myoglobin 16 KD and cytochrome C MW: 13 KD. Unknown protein MW: 400 KD, Catalase and MW:222 KD, Serum albumin MW:68 KD, myoglubin 16,000 D, Cytochrome C MW: 13,000 D

Explanation / Answer

Ans 15 d mobile phase

In mobile phase, ion exchange chromatography of proteins, buffers are used to bind proteins in column. Initially low concentration of salt is used, then it is increased to displace the proteins from stationary phase and elute from the column.

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