Use the micrograph of the Gram stain to draw conclusions and make predictions ab

Question

Use the micrograph of the Gram stain to draw conclusions and make predictions about the indicated bacterium. Based on your observation of the indicated bacterium in this Gram stain, select ALL appropriate statements. The indicated bacterium produces endotoxin (also known LPS) The indicated bacterium is a spirochete. The indicated bacterium is a bacillus The indicated bacterium has a strep formation The indicated bacterium is Gram-positive Eosin Methylene Blue (EMB) or MacConkey agar would NOT to good choices of media to grow the indicated bacterium. The indicated bacterium is Gram-negative

Explanation / Answer

The indicated bacterium produces endotoxins known as lipopolysaccharide. - Gram negative bacterium has a thick lipopolysaccaride with O- antigen in the outer membrane and induces immune response. This lipid causes the toxicity.

The indicated bacterium is a bacillus- it is a rod shaped bacterium and not genus Bacillus

The indicated bacterium is Gram negative- it has a very thin peptidoglycan layer and a very thick lipopolysaccharide layer in the cell wall. Due to this, it is pink in colour. Lack of thick peptidoglycan causes the crystal violet and iodine complex to be washed out after decolorisation . And the counter stain safranon stains the cell pink.

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