Separation of Chloroplast Pigments by Paper Chromatography, 5) What is the purpo

Question

Separation of Chloroplast Pigments by Paper Chromatography, 5) What is the purpose of paper chromatography in this experiment?
6) B:Refer to the diagram. -Which of the 3 labels is the same as the origin in the Rf equation above?
C: You will us "Alternative Procedure for Transferring Pigments onto Paper" in the box below if the diagram of the chromatography set up. Read the procedure. -What could you use besides the handle of a pair of scissors to transfer the pigment onto the chromatography paper?
5. In part II: Separation of Chloroplast Pigments by Paper Chromatography, what is the purpose of paper chromatography in this experiment? 6. In part II: A. What is the equation for calculating the Rf value? from Baseline troveuled by Solvent- B. Refer to the diagram. Which of the 3 labels is the same as the origin in the Rf equation above? Chromatographic strip C You will use Alternative Procedure for Transferring Pigments onto Paper in the box below the diagram of the chromatography set up. Read the procedure. What could you use besides the handle of a pair of scissors to transfer the pigment onto the chromatography paper? Chloroplast extract spot 6. In part III: Absorption Spectrum of Chloroplast Extract,what is the purpose of using a spectrophotometer in this exercise? Solvent 354

Explanation / Answer

5. Paper chromatography works as partition chromatography where the sample partitions between the stationary phase and the mobile phase based on its polarity/hydrophobicity. Based on different partition coefficient of different pigments in the chloroplast, they will travel differently on the paper and thus can be separated from each other.

6. B. ‘Chloroplast extract spot’ label is same as origin.

6. C. One can use a capillary to do spotting onto the paper.

6. Spectrophotometer can be used to illuminate the paper at specific wavelength where the chloroplast pigments can be detected at their characteristic absorption spectrum.

Related Questions

Navigate

• Browse (All)
• Browse S
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.