Guide to Thermo Scientific Protein Assay Kits and Reagents Assay Range (uglml) (
ID: 164721 • Letter: G
Question
Guide to Thermo Scientific Protein Assay Kits and Reagents Assay Range (uglml) (Sample Volume) (see Note 1) Important Substance Compatibilities Microplate Uniformity Pierce Protein Detection Tube Compatible Incompatible Assay Product (Amax) (see Note 4) Reducing agents, High 562 nm 20-2,000 20-2,000 Detergents Chelators 5-250 BCA Reducing 562 nm 125-2,000 125-2,000 Detergents Chelators (9w) Reducing agents Agent Compatible (see Note Micro BCA 562 nm 0,52 Reducing agents, High 240n Detergents Chelators 660 nm Assay 660 nm 25-2,000 50-2,000 Detergents, Ionic detergents, Low (10ul) Reducing agents; (unless IDCR is SDS sample buffer used, see Note 3) (see Note 3) Coomassie Plus 595 nm 100-1,500 100-1,500 Most reducing agents; Detergents Medium (7u) Chelators (35ul) 1.25 1-25 (500ul) (150 l) Coomassie 595 nm 100-1,500 100-1,500 Most reducing agents; Detergents (Bradford) (20ul) (5ul) Chelators 1-25 1.25 (500ul) (150 l) Modified Lowry 750 nm 10-1,500 10-1,500 SDS Most detergents. High Reducing agents, Chelators Notes 1. Tube: sample volume per 1 ml total assay volume for measurement in 1 cm cuvette, Microplate: sample volume per 200-300 ul total assay volume for measurementin 96-well microplate. 2, Levels typically used in cell lysis reagents and protein buffers. 3. Possible when the lonic Detergent Compatibility Reagent (IDCR) is used. 4. Protein-to-protein assay uniformity for a set of immunoglobulins, bovine serum albumin and other example proteins.Explanation / Answer
1. Human folate binding protein- assay ----> 660 nm assay. The buffer for this protein contains chelator, reducing agent and SDS (anionic detergent) . Thus the 660nm assay is the only assay that would be compatible for all the buffers that the protein would be dissolved in.
2. Human gamma globulin- assay ----> Micro BCA. The incompatibility of this assay does not include detergents and includes reducing agents and chelators, with compatibility of detergents. The buffer of this protein includes SDS which is a detergent, thus Micro BCA. This assay also has high uniformity.
3. Cytochrome C ( horse )-----> Coomassie Plus. Compatibility of the buffers include most reducing agents and chelators. The same compatibility is with Coomassie Bradford assay, but the uniformity is lower in Coomassie Bradford assay.
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