11) The base pair sequence of a region of a gene coding for a polypeptide is: St
ID: 164752 • Letter: 1
Question
11) The base pair sequence of a region of a gene coding for a polypeptide is:
Strand A: 5’ C A C C T A A C T T A C T C A G* C T T A C G A A G T C T A C C C A G C G 3’
Strand B: 3’ G T G G A T T G A A T G A G T C* G A A T G C T T C A G A T G G G T C G C 5’
A.) If this gene codes for a polypeptide that contains three Tyrosines (Tyr), which strand is transcribed? (1 pt)
B). What is the nucleotide of the mRNA transcribed from this DNA? (Note: there is no “start” codon. Simply start from the “appropriate” end). You MUST label the appropriate 5 ‘ and 3’ ends. (1 pt)
C.) What is the polypeptide sequence of the protein translated from this mRNA? (1 pt)
D.) Hydroxylamine (HA) affects the bold-faced (*) base pair and causes a mutation during DNA replication. What is the amino acid sequence of the polypeptide coded by the mutant gene? (1 pt)
Explanation / Answer
5’ CAC CTA ACT TAC TCA GCT TAC GAA GTC TAC CCA GCG 3’
3’ GTG GAT TGA ATG AGT CGA ATG CTT CAG ATG GGT CGC 5’
A. Strand that is transcribed is “
3’ GTG GAT TGA ATG AGT CGA ATG CTT CAG ATG GGT CGC 5’
B. Nucleotide of transcribed RNA is :
5’ CAC CUA ACU UAC UCA GCU UAC GAA GUC UAC CCA GCG 3’
C. Polypeptide translated is :
Histidine –Leucine-Threonine-Tyrosine-Serine—alanine-Tyrosine-Glutamic acid-Valine-Tyrosine-Proline-Alanine
D.
Mutated Strand
GTG GAT TGA ATG AGT C*GA ATG CTT CAG ATG GGT CGC 5’
Hyrdroxylamine causes transition of GC pair to AT pair : So next strand will be
3’ GTG GAT TGA ATG AGT TGA ATG CTT CAG ATG GGT CGC 5’
Transcribed RNA from Changed Strand:
CAC CUA ACU UAC UCA ACU UAC GAA GUC UAC CCA GCG 3’
Changed Polypeptide:
Histidine –Leucine-Threonine-Tyrosine-Serine—THREONINE-Tyrosine-Glutamic acid-Valine-Tyrosine-Proline-Alanine
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.