The following deals with DNA replication in bacteria. Match column A to Column B

Question

The following deals with DNA replication in bacteria. Match column A to Column B. The proteins in column B may be used more than once to their fuction in column . Each function in column A may have more than one answer, but you just need to pick one of them.

Column A Column B

unwinds the double helix A) DNA polmerase I

Keeps the DNA strands seperqated B) DNA polmerase III

Is an RNA polymerase C) HELICASE

Is an repair enzyme D)LIGASE

Is the major elogation enzyme E) SINGLE STRANDED BINDING PROTEIN

is a polymerase that makes the new DNA molecule in the 3'---> 5' direction F) NONE

Has only exonuclease activity G) PRIMASE

Bonds a 3' OH end of a polynucleotide to a free 5' nucleotide triphosphate H) TOPOISOMERASE

Removes RNA from the Okazaki fragments

Creates short primers for both template strands

forms covalent bonds between  Okazaki fragments

relieves positive supercoiling ahead of the replication fork

Explanation / Answer

Unwinds the double helix - Helicase

Keeps the DNA strand separated - Single stranded binding proteins

Is an RNA polymerase - Primase

Is an repair enzyme - Ligase and DNA polymerase I

Is the major elogation enzyme - DNA polymerase III

Is a polymerase that makes the new DNA molecule in the 3'---> 5' direction - DNA polmerase I

Has only exonuclease activity - DNA polmerase III

Removes RNA from the Okazaki fragments - DNA polymerase I

Creates short primers for both template strands - primase

forms covalent bonds between  Okazaki fragments - Ligase

relieves positive supercoiling ahead of the replication fork - Topoisomerase

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