HI please see the attached worksheet needed to complete questions 1-8. I need al
ID: 165211 • Letter: H
Question
HI please see the attached worksheet needed to complete questions 1-8. I need all #'s 1-8 questions completed please, thanks
Background You have identified a (fictional!) gene Q. This gene is thought to play a role in liking chocolate. You have the wild-type se- quence of this gene. In talking to acoworker, you learn that she detests chocolate, so you ask to sequence her gene Q. When you get the sequence back, you realize she has a mutation in this gene. 1. Based on what you know about the genetic code, would a mutation in a gene always lead to an altered protein? In this activity, you will transcribe and translate the wild-type gene Q, and then determine whether your coworker's mutation alters protein Q (and therefore whether it's possible that your coworker detests chocolate because of this mutation). Part 1: The pre-mRNA The sequence of the (fictionall gene Q is below. Here, the bottom strand is the template st 5'...TACTAATcGA AAATTGCAGA cTGTAATGGA GTCAGATGAT 3,....ATGATTAGcT TTTAACGTcT GACATTACcT CAGTcTACTA GCATTAAGGT AAGT.......A....TCAGG AGAAATTGAA ATAAA .3' cGTAATTCCA TTCA........T...AGTcc TCTTTAACTT TATTT...5' 2. write out the primary sequence of the RNA transcript starting at +1 and continuing until the end of the sequence provided in Box I on the last page of this handout. (write out the ..'s as well) "Hint: Have one group member read out the bases from the correct strand in the correct 5' 3' direction, and have another write them down.Explanation / Answer
We cannot answer more than one question per post. So I will answer the first question in the screenshot. Please post them in another post.
1. A mutation in a gene doesnt aways lead to an altered protein. There are many types of mutations. Some are silent mutations in which the mutated codon also codes for the same amino acid as the original codon. Some are nonsense codons where the mutated codon results in termination of the protein. These codons are usually stop codons.
Insertion of a base can also occur. Deletion of one or more bases can occur. These two types of mutations will lead to frameshift mutations where the reading frame is changed. More number of amino acids are mutated in this type of mutation.
A missense mutation is a mutation that changes the amino acid coded by the original codon to another amino acid. In such cases, an altered protein is seen.
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