A few amino acids are post-translationally removed from the C-terminal end of th

Question

A few amino acids are post-translationally removed from the C-terminal end of the beta-lactamase enzyme from B. imaginarium (i.e. - after it is translated and released from the ribosome, a protease chews off a few amino acids). The wild-type enzyme, which has had the amino acids removed from the C'-terminus, is 327 amino acids in length and the C-terminal amino acids are shown below aligned with the C-terminal amino acids of a frameshift mutant, which is not processed at its C-terminus. The wildtype and frameshift mutant have identical coding sequences except for the insertion or deletion of a single nucleotide in the sequences encoding the C-terminal region of the protein. Use this information to answer the questions below. AA#: 327 wild-type: Asn Met Asa Gly. Lys mutant: Asn Met lle Trp Gln Met Cys Asp Met Lys Asp What was the mutational event that gave rise to the frameshift mutant? (Identify which codon contains the inserted or deleted nucleotide and the position within the codon where the mutation occurred. Deduce the number of amino acids in the wild-type enzyme prior to processing of the C-terminus and, as far as possible, the actual sequence of the C-terminus of the wild-type enzyme

Explanation / Answer

Answer A. there will an insertion of U in the codon AAU which code for Asn. Insertion will make the AAU to AUA which codes for Ile.

Answer B. codon for the sequence before post processing in wild-type is:

AAU GGC AAA UGU UGA which code for

Asn Gly Lys Cys stop codon

Orin case of our protein, if the codon of Cys is UGC then

Asn Gly Lys Cys Arg Try stop codon

Related Questions

Navigate

• Browse (All)
• Browse A
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.