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Please answer 2a wing this method you set up each of four test tubes as below no

ID: 166489 • Letter: P

Question

Please answer 2a wing this method you set up each of four test tubes as below noted dATP, ITP, dGTP, dATP, dTTP. dOTP, dCTP dATP, aTTP dOTP. dCTP Alauber same stranded DAA and the and materials that are required for DNA replication are time period, you continue for the same length oftime in each the DNA from each and th ethidium bromide and observe the follow banding patterns on stration, we are using a DNA strand that is For this a. Which band in the gel contains the shortest DNA strand? What is the identity its terminal ddNTP? Integrate Your Understandingaareen 20 leotiles in DNA complement. of mac hod has since been modified so that each ddNTP used is Dow blue and ddATP. green. CTP, that you run the experiment that you did above. and adNTPv all the nucleotides (both on the following test tabe. You run the products of the reaction Integrate Your Understandi 20-2132

Explanation / Answer

The shortest DNA stand is the one which travels maximum in the agarose gel. In the given figure, lane 4 contains the band which travelled the longest among the four lanes.

As the lane 4 is ddCTP, the identify of terminal nucleotide is ddCTP.

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