We performed an experiment to find bacteriophage growth/ burst size. Nobody got

Question

We performed an experiment to find bacteriophage growth/ burst size. Nobody got good results that make sense, and our teacher asked us this.

"The experiment we performed to determine the average number of mature virions released did not work. We tried to syringe filter the virus before we infected the bacteria and then plated. Think about how we performed this experiment and offer an explanation as to why the experiment may not have worked."

I don't understand what went wrong. Should we not have syringe filtered the virus for some reason? Please help me understand!

Explanation / Answer

It can be due to the three reasons..

1) the filtration which is done may not be appropriate according to the size of the virion hence they are not filtered in the solution with which it is affected.. It means the filter size may be a bit problematic..

2) it is being observed that bacteriophage requires a certain ions in the media or buffers which helps them to attach to the bacteria cell hence the absence of those ions can be a leading reason for the no affected bacteria..!!

3) the another reason can be the bacteriophage may have attended the lysogenic phase whre I integrate into the bacteria insert its DNA into bacteria but do not Lysis the bacterial cells..!

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