When ligating your desired insert into the cloning vector, it is best to: a) hav

ID: 166947 • Letter: W

Question

When ligating your desired insert into the cloning vector, it is best to:

a) have more insert than vector in a blunt end ligation only to ensure that the insert is in the correct orientation.

b) have more insert than vector, regardless if it is blunt end or cohesive end ligation.

c) Have more insert than vector in a cohesive end ligation only because the vector is more prone to re-annealing with itself.

d) have more vector than insert so that the e.coli component cells will uptake the clone more readiliy.

There are mainly 3 conditions for effective DNA ligation:

Thus the correct option is option (C)

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