A researcher wants to use a spectrophotometer to determine the amount of a pigme

Question

A researcher wants to use a spectrophotometer to determine the amount of a pigment in a solution. The researcher will set the spectrophotometer to pass a beam of light at a specific wavelength through the sample. When set to display absorbance, the spectrophotometer will display a number that represents how much of the light was absorbed by the pigment molecules in the solution. Given this and the information below about how spectrophotometers work, which of the following are true? (Select ALL that apply.) HOW DOES A SPECTROPHOTOMETER WORK? Photoelectric Screen with a slit Pigment tube Record low absorption of Prism green light White light 1. Insert tube of 2. Instrument refracts 3. slitted screen is 4. Pigment sample 5. Photoelectric tube within purified pigment into a narrow beam of white moved to select a absorbs some the instrument converts light spectrophotometer. light with a prism. wavelength of light fraction of the light to electric current, displayed such as 525 nm remainder is by a meter. (green), to shine transmitted through the sample. Copyright 2008 Pearson Benjamin Cummings. All rights reserved. Low absorbance is recorded in the example O High absorbance is recorded in the example O Green pigments only absorb green wavelengths of light O The spectrophotometer can be adjusted to select a wavelength of light to shine through the sample O Most of the green light shined at the sample with green pigment is not absorbed

Explanation / Answer

Green pigments dont absorb green light, thats why they appear green.

Its shown that absorbance is low (in the image in question itself)

And we can choose the wavelength of light as per requirement in spectrophotometry.

Thus correct choices are:

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