This is the wild type sequence of the entire coding sequence of a gene: GGT ATG

Question

This is the wild type sequence of the entire coding sequence of a gene: GGT ATG GGG ACT TTGAGG ATGATA AGGCGT AAA TAA ATA T Which codes for the wild type protein sequence: Met-Gly-Thr-Leu-Arg-Met-lle-Arg-Arg-Lys What single mutation would change the protein sequence to Met-Gly-Thr-Leu-Arg-Gly? There might be more than one possibility. For the protein sequence: Met-Lys-Trp. Write out are all of the RNA strands that can encode the protein sequence. (There are six, a hint so you don't forget one of the important codons in this problem or the next.) Speed of transcription a. For a gene that is 4000 bp long, and an RNA polymerase speed of 40 bp/second, how long does it take to synthesize a single RNA molecule for this gene? You have this RNA fresh off the DNA template: 5' CGA, CGC CUC CCG UAU CCG CCA CGGACG CGC UGC CCC ACC nnn nnn nnn nnn UGA CUU UGA UAU GCA GCA AAA ACG GCU GAA UUU 3' The lower case n's indicate the middle of a long intron but not the ends of the intron, and the spacing is in threes to aid in writing the sequence. Using the rules for splicing, deduce exactly where the intron must be, write the exact sequence of the processed mRNA, and mark the border between exons. Which codons are susceptible to being converted to a "UAG" STOP codon with only a single nucleotide mutation?

Explanation / Answer

2. The mutation can be a change in the reading frame (i.e., a frameshift mutation), skipping 7 nucleotides in the forward direction and going directly to GGC, therefore coding for glycine instead of methionine.

3. AUG-AAA-UGG-UAA

AUG-AAA-UGG-UAG

AUG-AAA-UGG-UGA

AUG-AAG-UGG-UAA

AUG-AAG-UGG-UAG

AUG-AAG-UGG-UGA

There are 2 different codons for lysine and 3 different stop codons, so a total of 6 combinations of mRNA are possible.

4. 4000/40 = 100 seconds

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