Two probes designated probe A and probe B hybridize very near one another in a r

Question

Two probes designated probe A and probe B hybridize very near one another in a region of DNA that contains DNA fragment length variation when digested with the restriction enzyme HinIII. Four maps show the location and intervening distances in kilobases of HinIII restriction sites and the binding locations of probes A and B. The maps correspond to alles H1 and H4.

a) For the genotype H1H3, what DNA bands are expected in an autoradiogram using probe A? Using probe B? Using both probes together?

b) For the genotype H2H4, what DNA bands are expected in an autoradiogram using probe A? Using probe B? Using both probes together?

I hope my image posts so that you can answer the question but if not, I would appreciate even just some help getting to the right answer. I'm confused where to even start.

Explanation / Answer

ANSWER:

A) FOR THE GENOTYPE H1H3: DNA Bands are expected in an autoradiogram using probe A, Using probe B ,Using both probes together are

Using Probe A:4kb only

Using Probe B:3kb, 5kb

Using both Probe together: 3kb,4kb and 5kb

B) FOR THE GENOTYPE H2H4: DNA bands are expected in an autoradiogram using probe A, Using probe B,Using both probes together are

Using Probe A: 4kb

Using Probe B: 2kb, 8kb

Using both Probe together: 4kb, 2kb and 8kb

Related Questions

Navigate

• Browse (All)
• Browse T
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.