You gather kinetic data for an enzyme in the presence and absence of a new drug

Question

You gather kinetic data for an enzyme in the presence and absence of a new drug candidate. How does this drug affect the enzyme activity? A) It acts as a transition state analog. B) It acts as a non-competitive inhibitor. C) It acts as an allosteric inhibitor. D) It acts as a competitive inhibitor. E) It acts as an allosteric activator. What is the first step you would use to find the K_m of an enzyme with a new substrate molecule? A) Determine the V_max B) Plot how the initial velocity of the reaction changes with increasing concentration of substrate. C) Measure the rate of product formation (initial velocity) for many enzymatic reactions, each with a different concentration of substrate. D) Measure the concentration of products and reactants and calculate [products]/[reactants]. E) Calculate delta G degree' for the reaction.

Explanation / Answer

5. Correct option : b) It acts as a non-competitive inhibitor

Explanation: In this case Vmax is affected. So it is a non-competitive inhibitor. Because only non-competitive inhibitor change the Vmax.

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