Consider a bacterial promoter with -35 and -10 elements. What assay is best to s
ID: 168424 • Letter: C
Question
Consider a bacterial promoter with -35 and -10 elements. What assay is best to show that RNA polymerase binds at regions centered on the -35 and -10 positions upstream of the start site of transcription? (Reviewing Chapter 7 in the textbook may help.)
Consider a bacterial promoter with -35 and -10 elements. What assay is best to show that RNA polymerase binds at regions centered on the -35 and -10 positions upstream of the start site of transcription? (Reviewing Chapter 7 in the textbook may help.)
DNA footprinting assay chromatin immunoprecipitation assay electrophoretic mobility shift assay None of the above.Explanation / Answer
The correct answer is - chromatin immunoprecipitation assay .
The chromatin immunoprecipitation (ChIP) assay is a powerful and versatile technique used for probing protein-DNA interactions within the natural chromatin context of the cell. This assay can be used to identify multiple proteins associated with a specific region of the genome, or the opposite, to identify the many regions of the genome associated with a particular protein. In addition, the ChIP assay can be used to define the spatial and temporal relationship of a particular protein-DNA interaction. For example, the ChIP assay can be used to determine the specific order of recruitment of various protein factors to a gene promoter or to “measure” the relative amount of a particular histone modification across an entire gene locus during gene activation. In addition to histone proteins, the ChIP assay can also be used to analyze binding of transcription factors, transcription co-factors, DNA replication factors and DNA repair proteins.
Related Questions
drjack9650@gmail.com
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.