The DNA sequences encoding the initiation and termination codons of a certain pr

Question

The DNA sequences encoding the initiation and termination codons of a certain protein are separated by 6000 nucleotides on a certain organism's chromosome, however, when the mRNA obtained from this gene is translated, the protein product is only 300 amino acids in length. Assuming that there has been no post-translational processing of the protein, what can you conclude from these observations? A. the cell's ribosomes are not functioning correctly B. the RNA was synthesized in a bacterial cell C. the gene product in question is an integral membrane protein D. the RNA was synthesized in a eukaryotic cell E. nothing can be concluded from the above information When cells are grown in rich media, and therefore have high cytoplasmic levels of all amino acids, it can be assumed that all tRNAs are fully "charged" with their proper amino acids During the elongation phase of protein synthesis in such cells, an uncharged tRNA can NEVER occupy the ___ site of the ribosome A. E B. P C. A D. all of the above E. none of the above Which of the following types of virus involves a site-specific recombination in its interaction with its host cell? A. lytic virus B. lysogenic virus C. plant virus D. animal virus A mutation that blocks the activity of signal recognition particle (SRP) would be lethal because: A. DNA replication could not occur B. Transcription of mRNA would stop C. Translocation of proteins into the cell membrane and periplasmic space would be blocked D. Ribosomes would be unable to initiate translation of mRNA E. Biosynthesis of the cytoplasmic membrane would be blocked In Bacteria, translation of nascent RNA transcripts is often initiated before transcription is terminated. When this occurs: A. RNA is transcribed 3' rightarrow 5' and amino acids are added to the amino terminus of the growing polypeptide as the ribosome moves 3' rightarrow 5' along the mRNA B. RNA is transcribed 5' rightarrow 3' and amino acids are added to the amino terminus of the growing polypeptide as the ribosome moves 3' rightarrow 5' along the mRNA C. RNA is transcribed 3' rightarrow 5' and amino acids are added to the carboxy terminus of the growing polypeptide as the ribosome moves 5' rightarrow 3' along the mRNA D. RNA is transcribed 5' rightarrow 3' and amino acids are added to the carboxy terminus of the growing polypeptide as the ribosome moves 5' rightarrow 3' along the mRNA E. None of the above.

Explanation / Answer

26) all of the above is the answer.. It is because the t-Rna which is being bought is always loaded with the amino acids which forms the peptide hence the unloaded t-Rna can not binds to the site..

27) lysogenic viruses.. It is because they are the viruses which integrate their genome into the host genome and remain there for a longer period of time..!!

28) the answer is (C) translocation of protein will be block because the main purpose of the SRP is to transport the protein to mitochondria in eukaryotic and plasma membrane in prokaryotic cell hence the mutation in it can cause non transport hence creating a problem..!!

25) gene products is a integral membrane protein..

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