Refer to the following sequence of DNA: 5\' A T C G C G G T G 3\' 3\'TAGCGCCAC5\

Question

Refer to the following sequence of DNA: 5' A T C G C G G T G 3' 3'TAGCGCCAC5' a. If this DNA was replicated in E. coli in the presence of radioactive thymine (^3T) what replication products (2 products) will result (the symbol^3T instead of T if the Thymine is radioactive in the sequence). b. If the products above went through one more round of replication in radioactive thymine, what replication products will result (4 products). c. If the products above went through one more round of replication in normal thymine, what replication products will result (8 products).

Explanation / Answer

a)

DNAstrands: 5' - A T C G C G G T G - 3'

                       3' - T A G C G C C A C - 5'

DNA strands after replication: 5' - A T C G C G G T G - 3'

                                                    3' - 3T A G C G C C A C - 5'

Second strand:    3' - T A G C G C C A C - 5'

                                5' - A 3T C G C G G 3T G - 3'

b) DNA after 2nd round of replication: 5' - A 3T C G C G G 3T G - 3'

                                                                3' - 3T A G C G C C A C - 5'

3' - 3T A G C G C C A C - 5`

5' - A 3T C G C G G 3T G - 3'

Second strand: 5' - A 3T C G C G G 3T G - 3'

                               3' - 3T A G C G C C A C - 5'

3' - 3T A G C G C C A C - 5`

5' - A 3T C G C G G 3T G - 3'

c) DNA after 3rd round of replication:    5' - A 3T C G C G G 3T G - 3'

                                                                   3' - T A G C G C C A C - 5'

Second strand: 3' - 3T A G C G C C A C - 5'

                            5' -   A T C G C G G T G - 3'

5' -   A T C G C G G T G - 3'

3' - T A G C G C C A C - 5'

3' - T A G C G C C A C - 5'

5' -   A T C G C G G T G - 3'

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