Below the pl (isoelectric pH) the net charge of a peptide is positively charged

Question

Below the pl (isoelectric pH) the net charge of a peptide is positively charged and above the pl (isoelectric pH) the net charge of a peptide is negatively charged. At the pl a peptide or protein has a net charge of 0. Peptide A has a pl charged. of 5.5. (Choose all correct answers). a) at pH = 6.0 this protein will bind to an anion exchange column b) at pH 6.0 this protein will bind to a cation exchange column c) at pH 8.0 this protein will bind to an anion column d) at pH 8.0 this protein will bind to a cation exchange column A size exclusion (gel filtration) column composed of gel with fractionation range 50,000 - 500,000 Da is used to attempt to separate a mixture of proteins composed of A: 10,000 Da; B: 15,000 Da; C: 100,000 Da; D: 220,000 Da: E: 300,000 Da F: 400,000 Da What will be the order of elution of these proteins from this column? Choose the one best answer. a) First F and E will elute together in void volume, then D, then C then B then A will elute b) First A then B then C then D then E and F will elute last c) First B and A will elute first and together, then C then D then E then F d) First F then E then D then C and then A and B will elute together A mixture of proteins was subjected to SDS-Polyacrylamide electrophoresis. Which of the proteins would be expected to migrate the farthest during the electrophoresis? Choose the one best answer. a) Protein A: 10,000 Da b) Protein B: 15,000 Da c) Protein C: 100,000 Da d) Protein D: 220,000 Da e) Protein E: 300,000 Da

Explanation / Answer

The below two options are correct.

a) at pH 6.0 this protein will bind to an anion exchange column

c) at pH - 8.0 this protein will bind to an anion exchange column

As per the details given, since pH 6.0 and pH 8.0 is more than isoelectric pH 5.5 net charge will be negative (Anion). Since the charge is negative, ions are Anions. Anions will be bind to anion exchange column.

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