Gene sequence has the following elements A) Promoter sequence B) Transcription t

Question

Gene sequence has the following elements A) Promoter sequence B) Transcription terminator sequence C) Translation initiation sequence D) Translation termination sequence E) A and B F) All of the above For a given gene's promoter, are CAAT and TATA boxes on the coding strand or template strand? A mutation within a gene's sequence changes the start codon to a stop codon. How will this affect transcription of the gene? Explain your rationale. An RNA transcript has the following sequence: 5'-UACCGGAUGA-3' Write out the template DNA strand below On which side (5' or 3') of the template strand is the promoter located? List two similarities and one difference between DNA helicase used in DNA replication and p(rho) protein used in transcription If the following DNA strand were used as a template, what would the sequence of an RNA be? 5' GTACCGTC 3' A) 5' GUACCGUC 3' B) 5' GACGGTAC 3' C) 5' CAUGGCAG 3' D) 5' GACGGUAC 3' E) 5' GUCGGUAC 3'

Explanation / Answer

1. a gene consists of a promoter sequnce which marks the starting of transcrption, Transcription terminator site, translation start and stop sequence

2. For a gene, TATA box and CAAT are double stranded DNA entity. therefore, specifying its location on any one strand is meaningless.Usually we say TATA or CAAT by looking at the orientation ie.TATA and CAAT are located on the coding strand and their complementary sequence ATAT and GTTA are located on the template strand.

3. A mutation changing start codon to stop codon doesnt affect the transcription proceess as a whole. transcription will proceed producing a m-RNA with an altered sequnce (start codon, AUG changes to a stop codon)

4. Template strand sequence : 5'- TCATCCGGTA- 3'

Promoter site is located at the 3' end

5. similarities

Differences:

6. 5' GUACGUC 3'.

RNA polymerase synthesises RNA in 5' to 3' direction. the given DNA sequence is from 5' to 3'. therefore, the strand which is complementary to the given strand acts as the template and synthesizes RNA

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