Find a research article - perhaps on PubMed, or Google Scholar, or through the l

Question

Find a research article - perhaps on PubMed, or Google Scholar, or through the library, and copy and paste the abstract into your post.  You do not need to read the full article - just the abstract.  Deduce from this abstract the following:     1. The hypothesis of the study.   2. The use of scientific method and experimental design that you expect to see in this study based on just the information in the abstract.

Remember to finish with your opinion and impressions, in your own words, on how effective the abstract is.

Explanation / Answer

Please find the article below:

Title: A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding

Abstract: A protein determination method which involves the binding of Coomassie Brilliant Blue G-250 to protein is described. The binding of the dye to protein causes a shift in the absorption maximum of the dye from 465 to 595 nm, and it is the increase in absorption at 595 nm which is monitored. This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr. There is little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose. A small amount of color is developed in the presence of strongly alkaline buffering agents, but the assay may be run accurately by the use of proper buffer controls. The only components found to give excessive interfering color in the assay are relatively large amounts of detergents such as sodium dodecyl sulfate, Triton X-100, and commercial glassware detergents. Interference by small amounts of detergent may be eliminated by the use of proper controls.

1. Hypothesis of the study: Protein purification absolutely requires rapid and reproducible protein quantification assays. Presently available assays are time-consuming, have interference from cations and anions and have poor reproducibility. Hence there is requirement to develop rapid and reproducible assays. The fact that proteins bind irreversibly to coomassie brillian blue dye in a stoichiometric ratio can be utilized to develop a protein quantification assay.

2. The use of scientific method and experimental design: Analytical grade dxperimental reagents were procured for the study. Various test proteins were crytallized for evaluation of the assay. Protein standards were very carefully prepared. Absorbance of protein-dye complex was read at sensitive instrument and calculations were made.

Inference: The present study has outlined a very useful, rapid, reproducible and easy method for understanding alongwith qunatitative and qualitative evaluation of proteins in a sample. The abstract was very informative which effectively outlined the purpose of doing the study with brief description and results obtained. The conclusion was drawn without extrapolating the results and thus, the abstract was very concise and informative.

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