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place the steps for conducting the southern blot 3/25/2017 02:55 AM 58.9/100 G 3

ID: 170900 • Letter: P

Question

place the steps for conducting the southern blot 3/25/2017 02:55 AM 58.9/100 G 3/22/2017 0743 PM Print Calculator Periodic Table Question 29 of 40 Sapling Learning Mapd Place the steps for conducting the Southern blot technique in the correct order, from the isolation ofa DNA sample to the visualization of the target nucleotide sequence. ADNA sample is isolated from its souroe. The target mudeotde sequence is visualized using X-ray fan. The DNA is digested into smaller The DNA on the agarose gel onto a ntser membrane separated on The DNA fragments are an agarose gel by The target nudeoton gence Previous ® ove up vow solution 2 check Answer o Next a Pgup

Explanation / Answer

1. The DNA is digested into smaller fragments using a restriction enzyme.

2. The DNA fragments are separated on an agarose gel by electrophoresis.

3. The DNA fragments on the agarose gel are transferred onto a filter membrane.

4. The target nucleotide is labeled with a flourescent probe.