In chemical reactions involving enzymes, you will often be working with very sma

Question

In chemical reactions involving enzymes, you will often be working with very small volumes of reagents. This helps promote the reaction by keeping all of the reagents in a highly-concentrated form. It also helps you use less of expensive enzymes and other reagents. However, as a result, you will need to be able to prepare dilutions using smaller volumes than you have used this semester. You have a stock solution of DNA at 100 mu g/mu l (micrograms/microliter). What concentration (in mu g/mu l) would you have if you mixed 63 mu l of your stock solution with buffer and brought it to a final volume of 155 mu l? Answers cannot contain more than one decimal place. If your answer is a whole number, put .0 after the whole number.

Explanation / Answer

Answer: 40ug/uL

Explanation:

Stock = 100ug/uL

Dilution factor =aliquot/final volume= 63/155 =0.4

Final concentration= 100 ug/mL x 0.4 = 40ug/uL

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