Nutrient Broth Cultures Staphylococcus epidemidis Pseudomonas aeruginosa Procedu
ID: 171006 • Letter: N
Question
Nutrient Broth Cultures Staphylococcus epidemidis Pseudomonas aeruginosa Procedure: 1. Each team of four will culture all listed organisms. Assign each person a 2. different organism. organism for isolation on a plate of each of the six types of media. 3- assigned Label all plates well and place in the incubator for incubation at 37 C for 24-48 hours. esults: Indicate on the table below which organisms grew on which plates, the amount of growth occurring and other growth effects color, media changes, hemolysis, etc.) Escherchia TStaphylococcus Staphylococcus Pseudomona epidermidis Laeruginosa Nutrient agar PEA Blood agar ttt beta MacConkey pink colony in col. EMB M Black Mannitol Salt Verio medi Cetrimide PEAExplanation / Answer
7.a. No growth of staphylococcus aureus on macconkey agar indicates that it is a gram +ve organism
b.
Nutrient agar: creamcolor colonies
PEA: cream color colonies
Blood agar: large, round, golden-yellow colonies with Beta hemolysis
Macconkey: no growth
EMB: Small, circular colonies
Mannitol salt agar: colonies are colour less and media changes from lite pink to yellow.
Cetrimide: no growth
8. a.No growth of staphylococcus epidermis on macconkey agar indicates that it is a gram +ve organism
b.
Nutrient agar: creamcolor colonies
PEA: cream color colonies
Blood agar: alpha on blood agar
Macconkey: no growth hemolysis
EMB: Small, circular colonies
Mannitol salt agar: colonies are colour less and no change in colour of media.
Cetrimide: no growth
9.a. Growth of E.coli on emb and macconkey agar indicates that it is a gram -ve organism
b.
Nutrient agar: creamcolor colonies
PEA: growth is normal
Blood agar: Gamma-hemolytic (nonhemolytic) colonies on blood agar
Macconkey: no growth
EMB: very dark color colonies
Mannitol salt agar: colonies are colour less and no change in colour of media.
Cetrimide: no growth
10.a. Growth on cetrimide agar indicates it is p.aeroginosa.
b.
Nutrient agar: Growth is normal
PEA: growth is normal
Blood agar: Gamma-hemolytic (nonhemolytic) colonies on blood agar
Macconkey: colourless colonies
EMB: color less colonies
Mannitol salt agar: colonies are colour less and no change in colour of media.
Cetrimide: growth is observed
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