Calculate the RCF for a centrifuge with a rotor with a spinning radius of 6 cm t

Question

Calculate the RCF for a centrifuge with a rotor with a spinning radius of 6 cm that is spun at 4,000 RPM. Calculate the RPM for a centrifuge with a rotor with a spinning radius of 6 cm that needs a relative centrifugal force of 5,000 g. You are transferring the parameters from a centrifuge of radius 5 cm at a speed of 2,000 RPM, to another centrifuge of radius 8 cm. At what speed should you set the new centrifuge? The radius of the centrifuge rotor is 4 cm at the top of the tube holder on the rotor and 7 cm at the bottom of the tube holder. What is the RCT of both the top and bottom of the tube when spinning the centrifuge at 8,000 RPM? Calculate the sedimentation coefficient (in Svedbergs) for the apoferritin protein with a mass of 441 kDa and a frictional coefficient of 1.22 times 10^-7 g/s. Assume a density of 1 g/mL for water. Demonstrate that the three tube configuration (below) is balanced by adding up the forces in all directions Convert the following protocol,

Explanation / Answer

1.

RCF=1.12X R X (RPM/1000)^2 where R=radius

=1.12 X 6 X ( 4000/2000)^2= 1.12 X 6 X4=26.88 N

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