The stock solution of each PCR primer is 100 µM. A 1:10 dilution of this stock w

Q: The stock solution of each PCR primer is 100 µM. A 1:10 dilution of this stock w

Answer: What is the concentration of the working primer stock? Undiluted stock of PCR primer: 100uM 1:10 (Ten times dilution) Working stock stock= 1:10 dilution= 10uM What is the final concentration of each primer in the PCR reaction? 1uL of working stock (10uM) into 50 uL of PCR reaction means 1:50 dilution Dilution factor=1/50 Final conc of primer = 10uM x 1/50 =0.2uM

ID: 171260 • Letter: T

Question

The stock solution of each PCR primer is 100 µM. A 1:10 dilution of this stock was prepared to give the working primer stock used to set-up the PCR reaction. You used 1 µl of the working stock in a 50 µl PCR reaction. What is the concentration of the working primer stock? What is the final concentration of each primer in the PCR reaction? Show all units and calculations. Perform this calculation in your laboratory notebook and turn in the duplicate at the beginning of the next laboratory session.

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Answer:

What is the concentration of the working primer stock?

Undiluted stock of PCR primer: 100uM

1:10 (Ten times dilution)

Working stock stock= 1:10 dilution= 10uM

What is the final concentration of each primer in the PCR reaction?

1uL of working stock (10uM) into 50 uL of PCR reaction means 1:50 dilution

Dilution factor=1/50

Final conc of primer = 10uM x 1/50 =0.2uM

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The stock solution of each PCR primer is 100 µM. A 1:10 dilution of this stock w

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The stock solution of each PCR primer is 100 µM. A 1:10 dilution of this stock w

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