Xhol (TCGAG) cuts at 3478 (4 nt 5\' ext) What are the positions of BamHI and Xho

Question

Xhol (TCGAG) cuts at 3478 (4 nt 5' ext)

What are the positions of BamHI and XhoI digestion sites? How many fragments and how big are the fragments after digesting with only one enzyme? How many fragments and how big are the fragments after digesting with both enzymes? Show your calculation/explanation.

Sequence digested with: BamHI, Xhol Main options New custom digest View gel Print Custom Digest Circular Sequence: unnamed sequence ORFs a: 316 aan b: 264 aa c: 238 aa d: 137 aa e: 113 aa BareH 4751 bp XXho spla Linear Help Comments cleavage code Enzyme name code l blunt end cut Ava able from NEB Has other supplier l 5' extension Not commercially available cleavage affected by CpG reth. I 3' extension tt: cleavage affected by other meth. T l cuts 1 strand cenz.nare): ambiguou site s List Enzymes & sites Fragments

Explanation / Answer

BamHI digestion will result in a linear product of 4751bp as BamHI cuts the circular plasmid ones and produces a cleave leading to plasmid linearization.

XhoI digestion will result in a linear product of 4751bp as XhoI cuts the circular plasmid ones and produces a cleave leading to plasmid linearization.

Double digestion with BamHI and XhoI will produce 2 cuts/ nicks in the circular plasmid producing 2 products of sizes smaller fragment of 506bp and larger fragment of 4245 bp

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