Determine whether each of the following is a characteristic of DNA polymerase, R

Question

Determine whether each of the following is a characteristic of DNA polymerase, RNA polymerase, both or neither, Dependent on a RNA template A. Neither Polymerizes in the 5' rightarrow 3' direction B RNA polymerase only Requires deoxynucleotide triphosphate C. Both Dependent on a DNA template D. DNA polymerase only Has 3' rightarrow 5' exonuclease proofreading activity Uses adenine as a nitrogenous base Uses thymine as a nitrogenous base Catalyzes phosphodiester bond formation Requires a RNA primer

Explanation / Answer

1. Dependent on a RNA template - neither       

2. Polymerizes in the 5’ 3’ direction - both

3. Requires deoxynucleotide triphosphate - DNA polymerase only

4. Dependent on a DNA template - both

5. Has 3’ 5’ exonuclease proofreading activity – DNA polymerase only

6. Uses adenine as a nitrogenous base - neither

7. Uses thymine as a nitrogenous base – DNA polymerase only

            DNA has the base thymine while RNA has uracil as its base.

8. Catalyzes phosphodiester bond formation - both

9. Requires a RNA primer – DNA polymerase

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