1a and 1b please. Here is graph of describing what happened to a culture when it

Question

1a and 1b please.

Here is graph of describing what happened to a culture when it was exposed to a temperature higher than it's maximum temperature The following two questions are about using heat to kill microorganisms. What is the decimal reduction time for this organism? You repeat DRT experiment with a Gram-positive rod and this is the result. Your water bath maintained a constant temperature; your dilution and plating technique is perfect All the colony morphologies match that ove your original culture. What would cause this result?

Explanation / Answer

1 a) Decimal Reduction Time ( 90% reduction in population occurs) or D = t/ (Log N at o sec - Log N at t sec)

= 200/ (log 10^8 - log 10^7) = 200

1 b) We are not experiencing a steady reduction in population of the gram positive rods even after continuing to make the microbe experience heated waterbath. The primaryr eason is because the gram positive rod is an endospore forming bacteria, hence whatever population has survived are those which formed endospore and survived the adverse conditions created due to heat. So once we plate the culture, we provide the endospores the ideal criteria to regrow, hence a steady growth is observed.

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