this or more than one question but can you try helping me as much as possible A

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this or more than one question but can you try helping me as much as possible

A site is the specific location where a restriction enzyme (DNase) can cut the DNA. What are the number of Hpal site Two. Why? Looking at lane 2, the circular DNA must have been cut twice, since there are two bands of DNA. 2. What are the number of PstI sites? 3. What are the number of sspl sites? 4. Explain how you could determine that the total number of base pairs is 3900 if that number was not provided. 5. What is the purpose of the size standards (lane 1)? 6. Assume that each circle below represents the DNA asmid. Make cuts and label the band sizes pl to represent what each restriction (hydrolytic enzyme (or group of enzymes) did to the plasmid. The first one has been completed for you. Hin makes no difference where you draw the first cut, but the rest of the cuts are then dependent upon one another. 600 bp.

Explanation / Answer

Ans 1. DNAase cut the DNA sequence after recognition of the specific sites. They catalyse the hydrolytic cleavage of the phosphodiester linkages in DNA backbone, which degrades the DNA molecule. The diagram in the picture shows two HPA I sites where the DNA has been cleaved. So there were probably 2 recognition sequences in that circular DNA molecule.

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