Prior to knocking each gene down with RNAi, you use antibodies to determine the

Question

Prior to knocking each gene down with RNAi, you use antibodies to determine the wild-type expression pattern of the two novel proteins (NP1 and NP2) in relation to a-spectrin. You note that NP2 is localized to the entire cell cortex in a pattern similar to a- spectrin, but that NP1 is localized to only a subset of the cell cortex. Having determined the wild-type pattern for a-spectrin, NP1, and NP2, you then assay the expression of these three proteins when NP1 is knocked down with RNAi. In the 12 cells you are interested in, you drive expression of UAS-NP1 RNAi in two stripes (cells B1 /B2/B3 and D1/D2/D3). As before, you use antibodies to determine the expression pattern of NP1. NP2 and a-spectrin. You obtain these results. a spectrin novel protein (NPI) novel protein 2 (NP2) O Gal4 UAS-NP1 RNAi a-spectrin novel protein 1 (NP1) novel protein 2 (NP2)

Explanation / Answer

As RNAi is used for testing the expression of NP1 we found that:

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