During Drosophila development, adult structures are formed from clusters of cell

Question

During Drosophila development, adult structures are formed from clusters of cells called imaginal discs, which are recognizable during larval and pupal stages. You are interested in the genes expressed in imaginal discs, and you have identified two such genes that are closely linked together.

Gene 1 is expressed in the imaginal discs that will become the adult antennae.

Gene 2 is expressed in the discs that will become the adult wings and legs.

Part A You decide to make a construct that places the DNA between the genes upstream to a reporter gene (GFP) in place of Gene 1. The full-ength construct has the correct expression pattern in antennal discs. You then create several constructs that delete sections of the upstream DNA and score them for expression. The seven constructs are shown below. the black bars indicate the region(s) deleted in each construct. The data table on the right shows the expression results for each construct GFP GFP in GFP in GFP in antennal wing discs discs absent absent absent Construct 1 Construct 2 absent absent absent Construct 3 present present present absent absent Construct 4 present absent absent absent Construct 5 present present absent Construct 6 present absent absent Construct 7 From these results, you hypothesize that the sequence you have examined contains a promoter for gene 1 (P1), an enhancer that controls antennal expression (EA) an enhancer that controls leg and wing expression (EVM and an insulator region (I) Using the data, identify the function of each DNA segment by dragging the labels to their correct locations. Labels can be used once, more than once, or not at all.

Explanation / Answer

Basing on the construct provided and their expression, we can identify the DNA segment function correlated below

A: P1

B: Ea

C: I

D: EWL

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