In the laboratory, you decided to investigate the growth of Salmonella typhimuri

Question

In the laboratory, you decided to investigate the growth of Salmonella typhimurium in nutrient broth by using both viable cell counts and optical methods (spectrophotometry) simultaneously. Cultures of Salmonella were incubated in nutrient broth at 370C and aliquots were serially diluted and spread-plated on nutrient agar plates at indicated times. Simultaneously, the optical density of the culture was measured and indicated in the table below. Time (hrs) viable Counts (CFU/mL) OD 660nm 3.00 x 10 0.050 2.90 x 107 0.051 1.0 3.50 x 10 0.065 1.5 6.00 x 10 0.120 2.40 x 10 0.510 2.0 2,5 1.01 x 10 2.000 3.0 2.30 x 10 3.400 3.10 x 109 4.300 3.5 4.0 3.50 x 10 4.400 Plot the data using Excel and label all axes and clar all scales used. Submit the graph via email to your instructor. Indicate all growth phases observed for the Salmonella culture. Please ensure that you include your name in the Excel document as unnamed files will not be graded. You could use only one Excel file to send the two plots required for this assignment. Please also ensure that you include the data in the Excel document for reference. The latter indicates that you will have to place the date in the Excel file cells so that you could generate the graph. The two graphs must be generated on the same plot, that is, this will be a 3 axes graph. Please consult your supporting power point presentation to construct this particular graph with the indicated data. Remember that l will not consider this extra credit assignment for grading if you do not provide me the Excel file with the indicated growth curves and l will assign a "0" grade for the whole extra credit assignment.

Explanation / Answer

Part H) Cannot be determined with the information given.

Part I)   approximately 23 min/generation

Part J) approximately 23 min/generation

Part K) approximately 0.38 Hr/generation

Part L) approximately34 min/generation

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