HELP!!!!! Fermentation, one of the fates of pyruvic acid can be the production o

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HELP!!!!!

Fermentation, one of the fates of pyruvic acid can be the production of carbon dioxide and ethanol. The pyruvic acid is made from carbohydrate by glycolysis. By measuring the amount of CO_2 made over time, you can measure the efficiency of glycolysis. The basic experiment is relatively simple. Prepare a sample of carbohydrate in water. Take note of the concentration in % (use volume or weight). Completely fill a small juice glass with the carbohydrate mixture. Add yeast to the mixture, start with 1.4 tsp. Mix the carbohydrate, water, yeast mixture. If you spill some make sure the juice glass is filled to the brim. Place a flat-bottom bowl over the juice glass. Invert both the bowl and juice glass together, being careful not to spill the contents. Now the glass is upside down in the bowl. Watch the glass for at least 2 hours, recording the size of the bubble formed every 15 minutes. The size of the bubble can be estimated by measuring the height of the bubble in cm. Multiply the height by (1/4) times (diameter in cm) times (diameter in cm) x pi. Pi is approximate 3.14. (V = h times pi times r^2) Run this experiment 4 times (One experiment should be a control, using no carbohydrate. Vary only one variable. For example, one student might test sucrose (table sugar), Karo syrup, and honey. Another student might vary the concentration of carbohydrate; 5%, 10%, 15%. Another student might vary the temperature: in an ice bath, room temp, in a 100 degree F oven, design your own experiment.

Explanation / Answer

Fermentation is the metabolic process that takes place in the lower microorganism where in they convert sugars into different gases and alcohols under anaerobic conditions. Microorganisms perform fermentation to produce required energy (i.e. ATPs) under anaerobic conditions.

The production of Ethanol and carbondioxide vary depending on the type of the sugar involved, the concentration of the sugar and the temperature. Usually, the best temperature for fermentation process is the room temperature.

Experiments that can be performed to measure the amount of CO2 produced during the fermentation have been listed below. The student can choose any one of the experimental designs-

Experimental design 1 : Based on the type of the sugars-

Experimental design 2 : Based on the concentration of sugars-

Experimental design 3 : Based on the temperature-

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