Another application of the Polymerase Chain Reactor (PCR) is to screen samples f

Question

Another application of the Polymerase Chain Reactor (PCR) is to screen samples for the presence of viruses. For example, remember the recent Ebola outbreak? In the US, if a patient is suspected of having clinical symptoms and is at high risk for exposure to Ebola virus, one technique to determine if that person is infected with Ebola is to send a blood sample for PCR based analysis. In this assay, many copies of specific DNA sequences are made in order to cross a detection threshold. Each Ebola virus is composed of single strand of RNA approximately 18,060 nucleotides long enclosed within a cylindrical viral envelope. In some laboratories, the first atop might be to incubate samples with the enzyme Reverse Transcriptase. Why might this be? Choose all that apply. The polymerase chain reaction is identical to all transcription reactions. DNA m often the starting material for PCR so the viral genome is feat transcribed into RNA. DNA is often the starting material for PCR so the viral genome is first reverse transcribed into DNA. In PCR, RNA primers bind to specific regions of DNA not RNA so the RNA must first be converted to DNA.

Explanation / Answer

C. DNA is often the starting material for PCR so the viral genome RNA is first reverse transcribed into cDNA.

Polymerase obtain is used for replication or multiplication not for transcription, so only DNA is multiplied in PCR as it is double stranded and stable than RNA, no RNA primers is used

in RT PCR first RNA is reverse transcribed into cDNA and then amplified. It is used to measure the expression,Cancer detection, Ebola virus is RNA genome so it is easy to diagnosed by RT PCR method.

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