Primer design is a critical step for detection of viruses in a sample. PCR prime

Question

Primer design is a critical step for detection of viruses in a sample. PCR primers are short segments of DNA (about 20 nucleotides long) complimentary to the DNA that is being amplified. A pair of primers is designed and added to the sample. Which of the following would be the true requiring primers/primer design? Choose all that apply. PCR primers differ from primers produced in DNA replication within cells since they are composed of DNA not RNA RNA premise must be added to samples to create primers in a PCR reaction mix. The primers must not be complimentary to any regions of human RNA/DNA for detection of viral DNA. The viral genome must be known to effectively design primers. Primers are optional in PCR if you want to amplify the entire temple of DNA not just specific regions. Pairs of primers must be designed, one to start amplification with Taq polymerase and one with human polymerase. Pairs of primers must be designed, one for each side of the region of DNA to be amplified within a single DNA strand.

Explanation / Answer

PCR primers or polymerase chain reaction primer, is a short segment of DNA that researchers use to amplify, or replicate, a targeted portion of the DNA molecule. This results in billions of copies of the targeted area of DNA that enable scientists to conduct important research.

D)the viral genome should be known for effectively designed primer.

Related Questions

Navigate

• Browse (All)
• Browse P
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.