Shawn has decided to insert his salamanders with special bacteria that have an a

Question

Shawn has decided to insert his salamanders with special bacteria that have an added plasmid gene to produce titanium alloys. But first, Shawn needs to test a restriction enzyme to introduce a double-stranded break in the promoter of the Blue Glow (Bg) gene in the plasmid to add his own gene. When he finishes his transformations into the bacteria, he notices three different phenotypes. Using one-two sentences each, explain what might have happened in each of these bacteria

Bacteria with no color nor titanium:

Bacteria with glowing blue color but no titanium:

Bacteria with no color but have titanium:

Explanation / Answer

Ans: Bacteria with no color nor titanium: Means that there is not a succesful insertion and expression.

Bacteria with glowing blue color but no titanium: Means that the plasmid gene is not inserted and is not expressed. whereas the Bg gene has been succesful.

Bacteria with no color but have titanium: Means that the Bg gene is not expressed but the p;asmid gene is not expressed.

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