Give the fragment lengths that you would expect to result from the PCR and restr

Question

Give the fragment lengths that you would expect to result from the PCR and restriction enzyme digestion described in the lab manual for individuals with each of the following genotypes. Also, give their phenotype as "Taster" or "non-Taster". Given: What DNA sequence is recognized by the HaeIII enzyme? In the space below, give 3 to 5 short (-1 sentence each) bullet points describing, in your own words, what you will be doing in the lab today. There are a wide range of full-credit answers here; we will grade this as a 1-point check off.

Explanation / Answer

Please find the answers below:

Part 1: Phentylthiocarbamate or PTC is a chemical compound which can be tasted only by individuals with dominant genes for special taste buds on the tongue. Recessives cannot taste PTC hence are called non-tasters. Consider that the band for allele B and b are separately present in the genome. Thus, the details can be found as below:

Genotype Bands present Phenotype

BB A strong band for B allele Taster

Bb Two bands, for b and B each Taster

bb single band for b non-taster

Part 2: Restriction endonucleases are those enzymes who digest/cleave a target nucleic acid sequence at special points only. For enzyme HaeIII, the restriction digestion site is GGCC. The restriction site in this sequence is GG*CC (asterisked mark is site for digestion). This gives blunt end products with GG/CC sites on the DNA.

Part 3: It seems that this laboratory activity is targetted towards investigating tasters and non-taster individuals from the participants. Also, restriction digestion and agarose resolution of total DNA from various individuals can also be made to decipher genetic differences between these participants.

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