The conversion of 3-phosphoglycerate (3PG) to 2-phosphoglycerate (2PG) is an imp

Question

The conversion of 3-phosphoglycerate (3PG) to 2-phosphoglycerate (2PG) is an important cellular reaction within glycolysis. 3-phosphoglycerate doubleheadarrow 2-phosphoglycerate If the enzyme that catalyzes this conversion of 3PG to 2PG is added to a solution of 3PG at 25 degree C and a pH of 7.0, the Keq' 0.165. Experimental values for the actual steady-state concentrations of these compounds in human red blood cells are 61 mu M for 3PG and 4.3 mu M for 2PG. Constants: R = 1.987 cal/mol degree K Calculate the feta G degree for the conversion of 3-PG to 2-PG. Explain what this value means.

Explanation / Answer

delta G = -RT ln ( conc. of products/conc . of reactants )

= - 1.987 cal /mol K X (25+273) X ln (4.3/61)

= -1.987 X 298 X (-2.65) = 1563cal

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