In the lab, you use reverse transcriptase to synthesize DNA using an RNA templat

Question

In the lab, you use reverse transcriptase to synthesize DNA using an RNA template. For a eukaryotic gene, how will the DNA complementary to the mature mRNA compare to the genomic DNA from which the mRNA was originally transcribed? (Note: This complementary DNA is called cDNA)

A) The cDNA will contain ribose sugars, while the genomic DNA will contain deoxyribose sugars.

B) The cDNA will be identical to the transcribed region of the genomic DNA's coding strand.

C) The cDNA will include both exon and intron sequences, while the genomic DNA will not have intron sequences.

D) The cDNA will be identical to the transcribed region of the genomic DNA's template strand.

E) The cDNA will will not have intron sequences, while the genomic DNA will include both exon and intron sequences.

Explanation / Answer

The answer is E) The cDNA will not have intron sequences, while the genomic DNA will include both exon and intron sequences.

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