Protein purification and downstream processing Separation of a protein requires

Question

Protein purification and downstream processing Separation of a protein requires use of ammonium sulfate precipitation, an anion exchange column, and a hydrophobic interactions column. In which order would you perform this purification. Justify your strategy. In filtration, two of the most important experiments are generation of Flux v. TMP and Flux v. Concentration curves. Describe the measurement you would take to generate each of these curves. Note which parameters you are changing (you can control) and which you are measuring.

Explanation / Answer

The general procedure for the purifictaion of proteins is similar in almost all organisms. The basic procedure for purification of proteins are:

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