The following figure represents experimental results from study designed after t

Question

The following figure represents experimental results from study designed after the Beadle and Tatum experiment.

Assume the mutants are homozygous for recessive alleles causing their phenotypes.

a. If you crossed mutants 2 and 5, what would the nutrient requirements of the offspring be?

b. If you crossed mutants 2 and 4, what would the nutrient requirements of the F1 be? If you conducted an F1xF1 cross, what would the phenotypic ratios of the F2 be?

Does the nutrient support growth on minimal medium? Nutrient A Nutrient B Nutrient C Nutrient D Yes Mutant 1 Yes No Yes Yes Yes Mutant 2 No No Mutant 3 Yes Yes Yes Yes Mutant 4 No No Yes No Mutant 5 No Yes Yes No

Explanation / Answer

a). Both the mutants 2 and 5 are recessive for nutrient A and nutrient B. So, theory progeny will carry recessive mutation for these nutrients. Thus, their nutrient requirements will be nutrient C and nutrient D. Assuming that the genes are homozygous dominant.

b). Cross between 2 (CC DD) and 4 (CC dd) )will result in progeny with the nutrient requirements of nutrient C and nutrient D. The genotype of the F1 is, CC Dd

Self-cross of F1 gives the progeny with the following nutrient requirements,

CC Dd* CC Dd ---> CC D_ (3/4, require nutrient C and nutrient D), CC dd (1/4, requires nutrient C only)

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