1) What controls the frequency of switching the flagellar rotation in E. coli? A

Question

1) What controls the frequency of switching the flagellar rotation in E. coli?

A) the level of phosphorylated CheA

B) the level of phosphorylated CheY

C) the disulfide bridge in ArcB

D) export of the FlgM anti-sigma factor

E) none of the above

2) What controls the methylation state of methyl-accepting chemotaxis proteins?

A) the activity of CheB

B) the activity of CheB~P

C) the activity of CheR

D) the activity of both CheB~P and CheR

E) none of the above

3) What molecule is used to supply the methyl group that is transferred by many methyltransferases including CheR?

A) glutamate

B) glutamine

C) S-adenosyl homocystein

D) S-adenosyl methionine

E) none of the above

5) If a biofilm was capable of forming with E. coli (Ec) and Geobacter sulfurreducens (Gs), what metabolic scenario (electron flow) could support it?

A) glutamate -> Gs -> metal -> Ec -> O2

B) glutamate -> Ec -> H2 -> Gs -> glucose

C) glutamate -> Gs -> H2 -> Ec -> glucose

D) glucose -> Ec -> H2 -> Gs -> metal

E) none of the above

6)Unfolded amino acid chains can be degraded without external energy.

A) True

B) False

7) Proteins fold spontaneously, although not always into their biologically active forms.

A) True

B) False

8) How might a call down-regulate the levels of a protein without reducing transcription?

A) Express an adapter that delivers the protein to a protease.

B) Sequester the protein in an inactive complex.

C) Induce ribosome stalling during translation to recruit tmRNA.

D) A and C

E) A, B, and C

Explanation / Answer

1) A - The level of phosphorylated CheA

2) D - The activity of both CheB an CheR

3) A - glutamate

5) B -

6) FALSE

7) TRUE

8) D - both A and C

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