a. beta hemolysis b. antibiotic susceptibility test c. enteric bacteria d. pyelo

Question

a. beta hemolysis b. antibiotic susceptibility test c. enteric bacteria d. pyelonephritis e. medical mycology Use the following link or your lab manual to answer the following questions. htt www.protocol- forum ndex core&module-attach;& n attach&atta; ida 3626 Include details of tests done and what results would look like. 2) A sore throat epidemic broke out at a local school. How would you verify that the illness is strep throat?(10pts) 3) Develop a strategy for determining the causative agent of bacterial pneumonia.(25pts) 4) A strain of bacteria was isolated from a shipment of lettuce. After gram staining it was determined to be a gram negative rod. To further analyze the bacteria the Enterotube ll was inoculated. The results obtained showed positive reactions for glucose an gas production, lysine, ornithine,H2S, arabinose, sorbitol, and dulcitol. Using the given template and the code book should finding this organism be a concern?(10pts) ID Value 5) Discuss the three clinically relevant spirochetes discussed in lab.(10pts) 6) How would you diagnose tuberculosis(10pts) 7) What special requirements are needed to grow Clostridium in the lab? What is its clinical significance? (10pts)

Explanation / Answer

1) a) Beta hemolysis - is a complete breakdown of haemoglobin of RBC in the vicinity of bacterial colony. It is an indicative of a Streptococcus organisms.

Hemolysis is the breakdown of membrane of RBC by a bacterial protein known as hemolysin, which causes release of haemoglobin from RBC.

b) Antibiotic Susceptability Test - This test determines susceptability of a microbial species against different antibiotic agents. This varies within species and is usually carried to determine which antibiotic will be more successful in treating bacterial infection in vivo. AST mainly done by kirby-Bauer method.

c) Enteric Bacteria - Enteron means intestine. These are rod shaped gram negative bacteria occur normally in intestines of humans and other animals. They belongs to the family Enterobacteriaceae.These can be controlled by using antibiotics and other drugs which attack bacteria in gut.

Examples includes Faecalibacterium Prausnitzii, Lactobacillus,Enterococcus, E.coli, salmonella, Staphylococcus, Pseudomonas etc

d) Pyelonephritis - The inflammation of kidney due to specific type of urinary tract infection (UTI). The UTI begins in urethra or bladder and travels to kidneys. Fever, frequent urination and pain in the back side are symptoms. It is a serious kidney infection that can spread to blood and cause severe illness and are mostly curable with antibiotics.

Most common causes are bacteria from skin or environment, bacteria found in stool, reduced urine flow, kidney stones and diabetic conditions. It is diagnosed by physical examination, urinalysis.

e) Medical Mycology - Fungi has been recognised as disease causing even before bacteria. Most fungi are saprophytes, infections are opportunistic. Fungi are Eukaryotic protista having rigid cellwall with nuclear membrane.Fungi which forms mycelium is caled as moulds.

Based on morphology, divided into Yeasts - Cryptococus neoformans, Yeast like fungi - Candida Albicans, Moulds - Dermatophytes, Dimorphic fungi.

6) Tuberculosis is diagnosied by several tests like drug susceptibility tests, TB skin test, TB INterferon gamma release assays (IGRAs), Sputum smear microscopy test, chest x-ray and serological tests

7) Clostridium is a gram positive bacteria and are obligate anaerobes capable of producing endospores. They inhabit soils and intestinal tract of animals including humans and is normal inhabitant of healthy lower reproductive tract of women.

C. thermocellum generate ethanol and is thermophilic.

C.acetobutylum produces acetone and biobutanol from starch

C.botulinum produces diluted form of drug botux

C.histolyticum used as a source of enzyme collagenase

5) Relevant spirochetes are Leptospira species, Borrelia burgdorferi,Treponema pallidum, Brachyspira pilosicoli.

Related Questions

Navigate

• Browse (All)
• Browse A
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.