Specific recognition of DNAs by Cas9 is mediated by ___? specific protein-DNA in

Question

Specific recognition of DNAs by Cas9 is mediated by ___? specific protein-DNA interactions DNA-DNA Watson-Crick complementary base-paring RNA-DNA Watson-Crick complementary base-paring RNA-RNA Watson-Crick complementary base-paring Which of the statements about the CRISPR/Cas9 system is NOT true ___? Cas9 is an RNA-guided endonuclease. a flanking PAM motif in the target DNA is required for the system to recogni the DNA target. it makes double stranded blunt end cuts on the target DNA. the natural three components system can be simplified to a two components system containg Cas9 and sgRNA (without other cofactor) the natural system can also recognize and cut single stranded RNA. Describe the molecular mechanisms for the application of CRISPR/Cas9 as a DNA editing tool. Make sure to include the followings: minimal components of the system, role of each of the components, how the system achieve specific DNA recognition, what kinds of cuts are made, what intrinsic cellular mechanisms are responsible for DNA editing after DNA cleavage.

Explanation / Answer

29. A. Specific protien- DNA interaction

30.E.The natural system can also recognize and cut single stranded RNA .

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