Why are the \'sticky ends\' produced by restriction enzymes important to this pr

Question

Why are the 'sticky ends' produced by restriction enzymes important to this process? Al1 of these are reasons why sticky ends are important to the process of cloning a gene. The sticky ends allow the target DNA to complimentary base pair with the bacterial plasmid DNA. The sticky ends contain the enzyme ligase that 'glues' the target DNA to the bacterial plasmid DNA. The sticky ends allow a researcher to recognize the bacterial colony that contains the gene of interest. The sticky ends are the regions where the DNA is cut.

Explanation / Answer

Restriction enzymes are defined as enzyme which recognize a specific recognition sequences in DNA and then cut at this site to produce cohesive or blunt-ended DNA fragments. The various restriction enzymes used in genetic engineering are BamHl, PvuII, EcoR1, Kpnl, these act at specific sites in DNA.

There are two ways by which a DNA molecule can be cut that results in two types of ends in a DNA molecule:

Therefore, the correct option is: The sticky ends allow the target DAN to complimentary base pair with the bacterial plasmid DNA

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