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Which statement is correct? T-DNA insertion approach is a knock-out mechanism in

ID: 177214 • Letter: W

Question

Which statement is correct? T-DNA insertion approach is a knock-out mechanism in Arabidopsis that disrupts the gene of interest and the gene can no longer be transcribed into a functional mRNA. As such, this is an approach which works based on transcriptional regulation of gene expression. Talen technology is considered as forward genetic approach because it creates a new variation. In forward genetic approach, we determine the function of gene by analyzing the phenotypic effects of altered DNA sequences. T-DNA insertion approach is a knock-out mechanism in Arabidopsis that disrupts the gene of interest and the gene can no longer be transcribed into a functional mRNA. As such, this is an approach which works based on post-transcriptional regulation of gene expression.

Explanation / Answer

Ans: T-DNA insertion approach is knockout mechanism in Arabidopsis, based on transcriptional regulation of gene expression. So, option A is correct.